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Real-time PCR-system Eco 48

Cole-Parmer Ltd. (PCRmax) (422057)

Real-time PCR-system Eco 48
High specification, economically priced real-time thermocycler. The Eco 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ± 0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Eco 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:

-Validated volume per well: 5 to 20 µl
-High temperature uniformity of ± 0.1°C across the whole block instantly after every temperature change
-40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
-Genotype in 4 minutes with over 99% accuracy
-Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
-Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm)
-4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm)
-Data is always collected in all four filters for all wells
-Distinguishes 5000 and 10000 template copies with 99 percent confidence

Additional features:

-48-well block
-MIQE compliant
-400 analyte specific qPCR reagent kits available
-No calibration required
-Calibrated dyes: SYBR®, FAMTM, HEXTM, ROXTM, Cy®5.
-Additional dyes within the filter wavelength range are supported with no additional calibration required
-Unlimited software licence
-2 years warranty

Scope of supply:
Real-time PCR-system Eco 48, EcoControl and EcoStudy software, USB stick, 50 plates, 50 plate seals, power and Ethernet cable
Specifications
Temperature range: 30 to 100°C
Temperature stability: ±0.1°C
Temperature uniformity: ±0.1°C within 1sec
Average ramp rate: 5.5°C/sec
Dimensions (W x D x H): 345 x 310 x 320mm
Weight: 13.6kg

PN	Manuf. PN	Supplier		PK	Type									General	Price	Quantity	Status	Add
6320624	ECORT48	Cole-Parmer Ltd. (PCRmax)		1	Eco 48										28513.00 EU

Real-time PCR-system Prime Pro 48

Cole-Parmer Ltd. (Stuart) (2345)

Real-time PCR-system Prime Pro 48
The new Prime Pro 48 real time PCR system from Techne (UK) is a high specification, economically priced real-time thermocycler. The Prime Pro 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:
-Validated volume per well: 5 to 20 µl
-High temperature uniformity of ±0.1°C across the whole block instantly after every temperature change
-40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
-Genotype in 4 minutes with over 99% accuracy
-Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
-Dual LED excitation (452nm to 486nm and 542nm to 582nm)
-4 emission filters (505nm to 545nm, 562nm to 596nm, 604nm to 644nm, 665nm to 705nm)
-Data is always collected in all four filters for all wells
-Distinguishes 5000 and 10000 template copies with 99 percent confidence

Additional features:
-48-well block
-MIQE compliant
-400 analyte specific qPCR reagent kits available
-No calibration required
-Calibrated dyes: SYBR®, FAMTM, HEXTM, ROXTM, Cy®5.
-Additional dyes within the filter wavelength range are supported with no additional calibration required
-Unlimited software license
-1 year warranty

Custom 48-well qPCR plates and corresponding seals have to be ordered separately.
Specifications
Temperature range: 30 to 100°C
Temperature stability: ±0.1°C
Temperature uniformity: ±0.1°C within 1sec
Average ramp rate: 5.5°C/sec
Dimensions (W x D x H): 345 x 310 x 320mm
Weight: 13.6kg

PN	Manuf. PN	Supplier		PK	Type									General	Price	Quantity	Status	Add
9595516	93945-99	Cole-Parmer Ltd. (Stuart)		50	Plate seals Prime Pro 48										66.18 EU

Thermocycler Alpha Cycler

Cole-Parmer Ltd. (PCRmax) (3022)

Thermocycler Alpha Cycler
The Alpha Cycler family form PCRmax provides a range of features which are specifically designed to meet the needs of modern researchers when amplifying DNA. Given the flexibility in format and block options (96 or 384 well) scaleability is core in the Alpha Cycler range. Alpha Cycler software has features such as recently used programmes, allowing users to quickly access their most commonly used protocols without the need to navigate through folders to find it, individual user logins, with protected protocols. The Alpha Cycler systems also contain a novel programme wizard which allows users to define a protocol based specifically on their primer sequence and template source. Primer sequences or TM's are entered, amplicon length and source defined and the on board algorithm generates an optimised protocol for your assay. The Alpha Cyclers range of block options allow for use of the most commonly used consumables from different manufacturers and includes 96 well plates (0.2 ml), tubes (0.2 ml), strip tubes (0.2 ml) and 384 well plates.
-Single, dual or four block options available with either 96 or 384 well gradient blocks
-Multi-block units have totally independent blocks for maximum flexibility
-HD Android driven touchscreen interface for simplified programming
-Generate and send instrument reports to diagnose the health of your system and identify any issues before they impact users work
-Lock programs with user defined password
-Alpha Track mobile app allows remote monitoring of run progress

Alpha Track App (free of charge): Track the progress of runs and health of your system remotely via the mobile app
The Alpha Track Application is used in conjunction with your Alpha Cycler and allows users to:
-Remotely track the progress of runs
-Send a health report for diagnosis by the technical support team
-Be notified of any issues and prevent system failures before occurring
Specifications
Block temperature: 10 to 100 °C
Block uniformity at 55 °C: ±0.3 °C
Temperature accuracy at 55 °C: ±0.25 °C
Max. Heating rate: 3.4 °C/s

PN	Manuf. PN	Supplier	Description	Dimensions (W x D x H)~mm	PK	Type	Weight~kg	Price
4658855	AC196	Cole-Parmer Ltd. (PCRmax)	1 x 96 well block	430 x 260 x 200	1	AC196	12	6957.71 EU
4658856	AC1384	Cole-Parmer Ltd. (PCRmax)	1 x 384 well block	430 x 260 x 200	1	AC1384	12	6958.25 EU
4658857	AC296	Cole-Parmer Ltd. (PCRmax)	2 x 96 well blocks	470 x 535 x 330	1	AC296	30	15026.76 EU
4658858	AC2384	Cole-Parmer Ltd. (PCRmax)	2 x 384 well blocks	470 x 535 x 330	1	AC2384	30	15006.47 EU
4658859	AC2196	Cole-Parmer Ltd. (PCRmax)	1 x 96 + 1 x 384 well blocks	470 x 535 x 330	1	AC2196	30	14478.82 EU
4658860	AC496	Cole-Parmer Ltd. (PCRmax)	4 x 96 well blocks	700 x 535 x 330	1	AC496	45	28660.59 EU
4658861	AC4384	Cole-Parmer Ltd. (PCRmax)	4 x 384 well blocks	700 x 535 x 330	1	AC4384	45	28674.12 EU
4658862	AC4196	Cole-Parmer Ltd. (PCRmax)	1 x 96 + 3 x 384 well blocks	700 x 535 x 330	1	AC4196	45	27821.76 EU
4658863	AC4296	Cole-Parmer Ltd. (PCRmax)	2 x 96 + 2 x 384 well blocks	700 x 535 x 330	1	AC4296	45	27821.76 EU
4658864	AC4396	Cole-Parmer Ltd. (PCRmax)	3 x 96 + 1 x 384 well blocks	700 x 535 x 330	1	AC4396	45	28728.24 EU